Title |
Effects of platelet-rich plasma in a model of bovine endometrial inflammation in vitro
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Published in |
Reproductive Biology and Endocrinology, September 2016
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DOI | 10.1186/s12958-016-0195-4 |
Pubmed ID | |
Authors |
Maria Giovanna Marini, Claudia Perrini, Paola Esposti, Bruna Corradetti, Davide Bizzaro, Pietro Riccaboni, Eleonora Fantinato, Giuseppe Urbani, Giorgio Gelati, Fausto Cremonesi, Anna Lange-Consiglio |
Abstract |
Endometritis reduces fertility and is responsible for major economic losses in beef and dairy industries. The aim of this study was to evaluate an alternative therapy using platelet-rich plasma (PRP). PRP was tested in vivo, after bovine intrauterine administration, and in vitro on endometrial cells. Bovine endometrial cells were cultured until passage (P) 10 with 5 % or 10 % PRP. Effect of PRP on endometrial cell proliferation and on the expression of genes [prostaglandin-endoperoxide synthase 2 (COX2), tumor protein p53 (TP53), oestrogen receptors (ER-α and ER-β), progesterone receptor (PR) and c-Myc] involved in the regulation of oestrus cycle and fetal-maternal interaction were evaluated. Moreover, to evaluate the ability of PRP to counteract inflammation, 10 and 100 ng/ml of bacterial endotoxin lipopolysaccharide (LPS) were used to inflame endometrial cells in vitro for 1, 6, 12, 24 and 48 h. The expression of genes such as interleukin 1β (IL-1β), interleukin-8 (IL-8), inducible nitric oxide synthase (iNOS), prostaglandin-endoperoxide synthase 2 (COX2/PTGS2), and the release of PGE-2, IL-1β and IL-8 were evaluated. In vivo treatment with PRP increased the detection of PR. In vitro, 5 % PRP at passage 5 increased proliferation rate and induced a significant increase in the expression of all studied genes. Furthermore, the results revealed that 10 ng/ml of LPS is the most effective dose to obtain an inflammatory response, and that PRP treatment significantly down regulated the expression of pro-inflammatory genes. This study lays the foundations for the potential treatment of endometritis with PRP in vivo. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 93 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 14 | 15% |
Student > Ph. D. Student | 12 | 13% |
Student > Master | 12 | 13% |
Researcher | 6 | 6% |
Student > Doctoral Student | 5 | 5% |
Other | 14 | 15% |
Unknown | 30 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 15 | 16% |
Medicine and Dentistry | 14 | 15% |
Agricultural and Biological Sciences | 10 | 11% |
Biochemistry, Genetics and Molecular Biology | 9 | 10% |
Unspecified | 3 | 3% |
Other | 10 | 11% |
Unknown | 32 | 34% |