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Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site

Overview of attention for article published in Environmental Microbiome, February 2016
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Title
Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site
Published in
Environmental Microbiome, February 2016
DOI 10.1186/s40793-016-0137-y
Pubmed ID
Authors

Juliane Wissuwa, Runar Stokke, Anita-Elin Fedøy, Kjersti Lian, Arne Oskar Smalås, Ida Helene Steen

Abstract

Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 25%
Student > Ph. D. Student 9 20%
Student > Master 6 14%
Student > Bachelor 3 7%
Student > Postgraduate 2 5%
Other 3 7%
Unknown 10 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 27%
Agricultural and Biological Sciences 12 27%
Environmental Science 1 2%
Nursing and Health Professions 1 2%
Immunology and Microbiology 1 2%
Other 2 5%
Unknown 15 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2016.
All research outputs
#20,655,488
of 25,373,627 outputs
Outputs from Environmental Microbiome
#579
of 786 outputs
Outputs of similar age
#231,495
of 313,160 outputs
Outputs of similar age from Environmental Microbiome
#11
of 16 outputs
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.