No polar effects observed at phenotypic or protein levels on downstream genes! Local effects might be present at the mRNA level, consistent with previous data https://t.co/zRRADL6qba. Always consider polarity for any mutagenesis strategy, IMO
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RT @cisneros_lab: If you want to delete or inactivate an entire operon in E. coli, I suggest you use Cas12a as we did in our new paper. Pro…
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RT @cisneros_lab: If you want to delete or inactivate an entire operon in E. coli, I suggest you use Cas12a as we did in our new paper. Pro…
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RT @cisneros_lab: If you want to delete or inactivate an entire operon in E. coli, I suggest you use Cas12a as we did in our new paper. Pro…