Title |
Insights into biodiversity sampling strategies for freshwater microinvertebrate faunas through bioblitz campaigns and DNA barcoding
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Published in |
BMC Ecology and Evolution, April 2013
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DOI | 10.1186/1472-6785-13-13 |
Pubmed ID | |
Authors |
Brandon J Laforest, Amanda K Winegardner, Omar A Zaheer, Nicholas W Jeffery, Elizabeth E Boyle, Sarah J Adamowicz |
Abstract |
Biodiversity surveys have long depended on traditional methods of taxonomy to inform sampling protocols and to determine when a representative sample of a given species pool of interest has been obtained. Questions remain as to how to design appropriate sampling efforts to accurately estimate total biodiversity. Here we consider the biodiversity of freshwater ostracods (crustacean class Ostracoda) from the region of Churchill, Manitoba, Canada. Through an analysis of observed species richness and complementarity, accumulation curves, and richness estimators, we conduct an a posteriori analysis of five bioblitz-style collection strategies that differed in terms of total duration, number of sites, protocol flexibility to heterogeneous habitats, sorting of specimens for analysis, and primary purpose of collection. We used DNA barcoding to group specimens into molecular operational taxonomic units for comparison. |
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Mendeley readers
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