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Integration of mate pair sequences to improve shotgun assemblies of flow-sorted chromosome arms of hexaploid wheat

Overview of attention for article published in BMC Genomics, April 2013
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  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

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Title
Integration of mate pair sequences to improve shotgun assemblies of flow-sorted chromosome arms of hexaploid wheat
Published in
BMC Genomics, April 2013
DOI 10.1186/1471-2164-14-222
Pubmed ID
Authors

Tatiana Belova, Bujie Zhan, Jonathan Wright, Mario Caccamo, Torben Asp, Hana Šimková, Matthew Kent, Christian Bendixen, Frank Panitz, Sigbjørn Lien, Jaroslav Doležel, Odd-Arne Olsen, Simen R Sandve

Abstract

The assembly of the bread wheat genome sequence is challenging due to allohexaploidy and extreme repeat content (>80%). Isolation of single chromosome arms by flow sorting can be used to overcome the polyploidy problem, but the repeat content cause extreme assembly fragmentation even at a single chromosome level. Long jump paired sequencing data (mate pairs) can help reduce assembly fragmentation by joining multiple contigs into single scaffolds. The aim of this work was to assess how mate pair data generated from multiple displacement amplified DNA of flow-sorted chromosomes affect assembly fragmentation of shotgun assemblies of the wheat chromosomes.

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The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 2 4%
United States 1 2%
France 1 2%
Argentina 1 2%
Unknown 52 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 37%
Student > Ph. D. Student 9 16%
Student > Bachelor 4 7%
Student > Postgraduate 4 7%
Student > Doctoral Student 3 5%
Other 9 16%
Unknown 7 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 35 61%
Biochemistry, Genetics and Molecular Biology 8 14%
Computer Science 2 4%
Unknown 12 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 April 2013.
All research outputs
#13,380,993
of 22,703,044 outputs
Outputs from BMC Genomics
#4,978
of 10,624 outputs
Outputs of similar age
#106,779
of 199,687 outputs
Outputs of similar age from BMC Genomics
#58
of 123 outputs
Altmetric has tracked 22,703,044 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,624 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 199,687 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 123 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.