Title |
A novel system for in situ determination of heat tolerance of plants: first results on alpine dwarf shrubs
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Published in |
Plant Methods, March 2013
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DOI | 10.1186/1746-4811-9-7 |
Pubmed ID | |
Authors |
Othmar Buchner, Matthias Karadar, Ines Bauer, Gilbert Neuner |
Abstract |
Heat stress and heat damage to plants gain globally increasing importance for crop production and plant survival in endangered habitats. Therefore the knowledge of heat tolerance of plants is of great interest. As many heat tolerance measurement procedures require detachment of plants and protocols expose samples to various heat temperatures in darkness, the ecological relevance of such results may be doubted. To overcome these constraints we designed a novel field compatible Heat Tolerance Testing System (HTTS) that opens the opportunity to induce controlled heat stress on plants in situ under full natural solar irradiation. Subsequently, heat tolerance can be evaluated by a variety of standard viability assays like the electrolyte leakage test, chlorophyll fluorescence measurements and visual assessment methods. Furthermore, recuperation can be studied under natural environmental conditions which is impossible when detached plant material is used. First results obtained on three alpine dwarf - shrubs are presented. |
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