Title |
Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection
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Published in |
Veterinary Research, September 2022
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DOI | 10.1186/s13567-022-01084-3 |
Pubmed ID | |
Authors |
Nigeer Te, Jordi Rodon, Rhea Creve, Mónica Pérez, Joaquim Segalés, Júlia Vergara-Alert, Albert Bensaid |
Abstract |
Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Germany | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 1 | 25% |
Social Sciences | 1 | 25% |
Unknown | 2 | 50% |