Title |
Elucidation of the glycosylation steps during biosynthesis of antitumor macrolides PM100117 and PM100118 and engineering for novel derivatives
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Published in |
Microbial Cell Factories, November 2016
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DOI | 10.1186/s12934-016-0591-7 |
Pubmed ID | |
Authors |
Raúl García Salcedo, Carlos Olano, Rogelio Fernández, Alfredo F. Braña, Carmen Méndez, Fernando de la Calle, José A. Salas |
Abstract |
Antitumor compounds PM100117 and PM100118 are glycosylated polyketides derived from the marine actinobacteria Streptomyces caniferus GUA-06-05-006A. The organization and characterization of the PM100117/18 biosynthesis gene cluster has been recently reported. Based on the preceding information and new genetic engineering data, we have outlined the pathway by which PM100117/18 are glycosylated. Furthermore, these genetic engineering experiments have allowed the generation of novel PM100117/18 analogues. Deletion of putative glycosyltranferase genes and additional genes presumably involved in late biosynthesis steps of the three 2,6-dideoxysugars appended to the PM100117/18 polyketide skeleton, resulted in the generation of a series of intermediates and novel derivatives. Isolation and identification of the novel compounds constitutes an important contribution to our knowledge on PM100117/18 glycosylation, and set the basis for further characterization of specific enzymatic reactions, additional genetic engineering and combinatorial biosynthesis approaches. |
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Spain | 1 | 100% |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 12 | 100% |
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Student > Postgraduate | 2 | 17% |
Student > Ph. D. Student | 2 | 17% |
Student > Bachelor | 1 | 8% |
Professor > Associate Professor | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
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Economics, Econometrics and Finance | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |