Title |
Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription
|
---|---|
Published in |
Journal of Nanobiotechnology, April 2013
|
DOI | 10.1186/1477-3155-11-8 |
Pubmed ID | |
Authors |
Igor D Vilfan, Yu-Chih Tsai, Tyson A Clark, Jeffrey Wegener, Qing Dai, Chengqi Yi, Tao Pan, Stephen W Turner, Jonas Korlach |
Abstract |
Zero-mode waveguides (ZMWs) are photonic nanostructures that create highly confined optical observation volumes, thereby allowing single-molecule-resolved biophysical studies at relatively high concentrations of fluorescent molecules. This principle has been successfully applied in single-molecule, real-time (SMRT®) DNA sequencing for the detection of DNA sequences and DNA base modifications. In contrast, RNA sequencing methods cannot provide sequence and RNA base modifications concurrently as they rely on complementary DNA (cDNA) synthesis by reverse transcription followed by sequencing of cDNA. Thus, information on RNA modifications is lost during the process of cDNA synthesis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 35% |
Canada | 2 | 12% |
United Kingdom | 2 | 12% |
Norway | 1 | 6% |
Unknown | 6 | 35% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 8 | 47% |
Members of the public | 8 | 47% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | <1% |
United Kingdom | 1 | <1% |
Norway | 1 | <1% |
South Africa | 1 | <1% |
Unknown | 219 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 53 | 24% |
Researcher | 46 | 21% |
Student > Master | 22 | 10% |
Student > Bachelor | 14 | 6% |
Professor > Associate Professor | 9 | 4% |
Other | 26 | 12% |
Unknown | 54 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 63 | 28% |
Biochemistry, Genetics and Molecular Biology | 57 | 25% |
Chemistry | 11 | 5% |
Medicine and Dentistry | 9 | 4% |
Immunology and Microbiology | 6 | 3% |
Other | 20 | 9% |
Unknown | 58 | 26% |