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Overview of attention for article published in BMC Cell Biology, January 2002
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Published in
BMC Cell Biology, January 2002
DOI 10.1186/1471-2121-3-3
Pubmed ID

Ken Scott, Jialiang Zhang


Previous work, by us and others, has shown that mammalian galectins-1 have a growth-inhibitory activity for mammalian cells which is apparently independent of their beta-galactoside binding site. We have made recombinant human galectin-1 as a bacterial fusion protein with an N-terminal hexahistidine tag. This protein displays both haemagglutination and growth-inhibitory activities, even in the presence of the hexahistidine tag. Site-directed mutagenesis of this protein has confirmed the independent nature of the protein sites responsible for the two biological activities. Mutant proteins were created, which displayed each activity in the absence of the other. Human galectin-1 possesses a growth-inhibitory site, which is not part of the beta-galactoside binding site. A surface loop, comprising amino acid residues 25-30, and joining two internal beta-strands, forms part of the growth-inhibitory site. This region is relatively close to the N-terminus of the protein, and N-terminal substitutions or extensions also affect growth-inhibitory activity. Further experiments will be necessary to fully define this site.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Argentina 1 10%
Unknown 9 90%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 40%
Student > Ph. D. Student 3 30%
Professor 1 10%
Student > Master 1 10%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 60%
Chemistry 2 20%
Unknown 2 20%