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Mendeley readers
Attention Score in Context
| Title |
MicroRNA-125b regulates the expression of aggrecanase-1 (ADAMTS-4) in human osteoarthritic chondrocytes
|
|---|---|
| Published in |
Arthritis Research & Therapy, February 2013
|
| DOI | 10.1186/ar4164 |
| Pubmed ID | |
| Authors |
Tetsuya Matsukawa, Tadahiro Sakai, Tomo Yonezawa, Hideki Hiraiwa, Takashi Hamada, Motoshige Nakashima, Yohei Ono, Shinya Ishizuka, Hiroyuki Nakahara, Martin K Lotz, Hiroshi Asahara, Naoki Ishiguro |
| Abstract |
INTRODUCTION: Increased expression of aggrecanase-1 (ADAMTS-4) has emerged as an important factor in osteoarthritis (OA) and other joint diseases. This study aimed to determine whether the expression of ADAMTS-4 in human chondrocytes is regulated by miRNA. METHODS: MiRNA targets were identified using bioinformatics. Chondrocytes were isolated from knee cartilage and treated with interleukin-1 beta (IL-1β). Gene expression was quantified using TaqMan assays and protein production was determined by immunoblotting. Luciferase reporter assay was used to verify interaction between miRNA and target messenger RNA (mRNA). RESULTS: In silico analysis predicted putative target sequence of miR-125b on ADAMTS-4. MiR-125b was expressed in both normal and OA chondrocytes, with significantly lower expression in OA chondrocytes than in normal chondrocytes. Furthermore, IL-1β-induced upregulation of ADAMTS-4 was suppressed by overexpression of miR-125b in human OA chondrocytes. In the luciferase reporter assay, mutation of the putative miR-125b binding site in the ADAMTS-4 3'UTR abrogated the suppressive effect of miR125. CONCLUSIONS: Our results indicate that miR-125b plays an important role in regulating the expression of ADAMTS-4 in human chondrocytes and this identifies miR-125b as a novel therapeutic target in OA. |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 3% |
| Japan | 1 | 2% |
| Spain | 1 | 2% |
| Italy | 1 | 2% |
| Unknown | 57 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 31% |
| Researcher | 10 | 16% |
| Student > Bachelor | 7 | 11% |
| Student > Master | 5 | 8% |
| Lecturer | 2 | 3% |
| Other | 7 | 11% |
| Unknown | 12 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 23% |
| Medicine and Dentistry | 12 | 19% |
| Biochemistry, Genetics and Molecular Biology | 11 | 18% |
| Veterinary Science and Veterinary Medicine | 3 | 5% |
| Engineering | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 12 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2013.
All research outputs
#17,286,379
of 25,374,647 outputs
Outputs from Arthritis Research & Therapy
#2,536
of 3,381 outputs
Outputs of similar age
#197,186
of 296,588 outputs
Outputs of similar age from Arthritis Research & Therapy
#31
of 44 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,381 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.2. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 296,588 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.