Title |
Cellular toxicity following application of adeno-associated viral vector-mediated RNA interference in the nervous system
|
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Published in |
BMC Neuroscience, February 2010
|
DOI | 10.1186/1471-2202-11-20 |
Pubmed ID | |
Authors |
Erich M Ehlert, Ruben Eggers, Simone P Niclou, Joost Verhaagen |
Abstract |
After a spinal cord lesion, axon regeneration is inhibited by the presence of a diversity of inhibitory molecules in the lesion environment. At and around the lesion site myelin-associated inhibitors, chondroitin sulfate proteoglycans (CSPGs) and several axon guidance molecules, including all members of the secreted (class 3) Semaphorins, are expressed. Interfering with multiple inhibitory signals could potentially enhance the previously reported beneficial effects of blocking single molecules. RNA interference (RNAi) is a tool that can be used to simultaneously silence expression of multiple genes. In this study we aimed to employ adeno-associated virus (AAV) mediated expression of short hairpin RNAs (shRNAs) to target all Semaphorin class 3 signaling by knocking down its receptors, Neuropilin 1 (Npn-1) and Neuropilin 2 (Npn-2). |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 1% |
Germany | 1 | 1% |
Luxembourg | 1 | 1% |
Unknown | 85 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 25% |
Student > Ph. D. Student | 16 | 18% |
Student > Bachelor | 10 | 11% |
Student > Doctoral Student | 8 | 9% |
Student > Master | 7 | 8% |
Other | 14 | 16% |
Unknown | 11 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 31 | 35% |
Neuroscience | 12 | 14% |
Biochemistry, Genetics and Molecular Biology | 11 | 13% |
Medicine and Dentistry | 11 | 13% |
Engineering | 3 | 3% |
Other | 6 | 7% |
Unknown | 14 | 16% |