Title |
Non-canonical protein-DNA interactions identified by ChIP are not artifacts
|
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Published in |
BMC Genomics, April 2013
|
DOI | 10.1186/1471-2164-14-254 |
Pubmed ID | |
Authors |
Richard P Bonocora, Devon M Fitzgerald, Anne M Stringer, Joseph T Wade |
Abstract |
ChIP-chip and ChIP-seq are widely used methods to map protein-DNA interactions on a genomic scale in vivo. Waldminghaus and Skarstad recently reported, in this journal, a modified method for ChIP-chip. Based on a comparison of our previously-published ChIP-chip data for Escherichia coli σ32 with their own data, Waldminghaus and Skarstad concluded that many of the σ32 targets identified in our earlier work are false positives. In particular, we identified many non-canonical σ32 targets that are located inside genes or are associated with genes that show no detectable regulation by σ32. Waldminghaus and Skarstad propose that such non-canonical sites are artifacts, identified due to flaws in the standard ChIP methodology. Waldminghaus and Skarstad suggest specific changes to the standard ChIP procedure that reportedly eliminate the claimed artifacts. |
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