Title |
Discovering motifs that induce sequencing errors
|
---|---|
Published in |
BMC Bioinformatics, April 2013
|
DOI | 10.1186/1471-2105-14-s5-s1 |
Pubmed ID | |
Authors |
Manuel Allhoff, Alexander Schönhuth, Marcel Martin, Ivan G Costa, Sven Rahmann, Tobias Marschall |
Abstract |
Elevated sequencing error rates are the most predominant obstacle in single-nucleotide polymorphism (SNP) detection, which is a major goal in the bulk of current studies using next-generation sequencing (NGS). Beyond routinely handled generic sources of errors, certain base calling errors relate to specific sequence patterns. Statistically principled ways to associate sequence patterns with base calling errors have not been previously described. Extant approaches either incur decisive losses in power, due to relating errors with individual genomic positions rather than motifs, or do not properly distinguish between motif-induced and sequence-unspecific sources of errors. |
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United Kingdom | 1 | 9% |
Venezuela, Bolivarian Republic of | 1 | 9% |
Norway | 1 | 9% |
Unknown | 2 | 18% |
Demographic breakdown
Type | Count | As % |
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Scientists | 8 | 73% |
Members of the public | 3 | 27% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 6 | 4% |
United Kingdom | 4 | 3% |
Italy | 2 | 1% |
Germany | 2 | 1% |
France | 1 | <1% |
Sweden | 1 | <1% |
Unknown | 121 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 45 | 33% |
Student > Ph. D. Student | 21 | 15% |
Student > Master | 18 | 13% |
Other | 10 | 7% |
Professor > Associate Professor | 7 | 5% |
Other | 19 | 14% |
Unknown | 17 | 12% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 68 | 50% |
Biochemistry, Genetics and Molecular Biology | 18 | 13% |
Computer Science | 13 | 9% |
Medicine and Dentistry | 4 | 3% |
Mathematics | 3 | 2% |
Other | 9 | 7% |
Unknown | 22 | 16% |