Title |
Massively-parallel sequencing of genes on a single chromosome: a comparison of solution hybrid selection and flow sorting
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Published in |
BMC Genomics, April 2013
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DOI | 10.1186/1471-2164-14-253 |
Pubmed ID | |
Authors |
Jamie K Teer, Jennifer J Johnston, Sarah L Anzick, Marbin Pineda, Gary Stone, NISC Comparative Sequencing Program1, Paul S Meltzer, James C Mullikin, Leslie G Biesecker |
Abstract |
Targeted capture, combined with massively-parallel sequencing, is a powerful technique that allows investigation of specific portions of the genome for less cost than whole genome sequencing. Several methods have been developed, and improvements have resulted in commercial products targeting the human or mouse exonic regions (the exome). In some cases it is desirable to custom-target other regions of the genome, either to reduce the amount of sequence that is targeted or to capture regions that are not targeted by commercial kits. It is important to understand the advantages, limitations, and complexity of a given capture method before embarking on a targeted sequencing experiment. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Germany | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Switzerland | 1 | 2% |
Netherlands | 1 | 2% |
Chile | 1 | 2% |
Australia | 1 | 2% |
China | 1 | 2% |
United States | 1 | 2% |
Unknown | 38 | 86% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 15 | 34% |
Student > Ph. D. Student | 8 | 18% |
Student > Doctoral Student | 5 | 11% |
Professor > Associate Professor | 3 | 7% |
Other | 2 | 5% |
Other | 8 | 18% |
Unknown | 3 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 28 | 64% |
Biochemistry, Genetics and Molecular Biology | 6 | 14% |
Medicine and Dentistry | 4 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Unknown | 5 | 11% |