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Mendeley readers
| Title |
Comparison of activity indexes for recognizing enzyme mutants of higher activity with uricase as model
|
|---|---|
| Published in |
BMC Chemistry, April 2013
|
| DOI | 10.1186/1752-153x-7-69 |
| Pubmed ID | |
| Authors |
Juan Feng, Hongbo Liu, Xiaolan Yang, Ang Gao, Juan Liao, Liping Feng, Jun Pu, Yanling Xie, Gaobo Long, Yuanli Li, Fei Liao |
| Abstract |
For screening a library of enzyme mutants, an efficient and cost-effective method for reliable assay of enzyme activity and a decision method for safe recognition of mutants of higher activity are needed. The comparison of activity concentrations of mutants in lysates of transformed Escherichia coli cells against a threshold is unsafe to recognize mutants of higher activity due to variations of both expression levels of mutant proteins and lysis efficiency of transformed cells. Hence, by a spectrophotometric method after verification to measure uricase activity, specific activity calculated from the level of total proteins in a lysate was tested for recognizing a mutant of higher activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 20% |
| Student > Ph. D. Student | 3 | 15% |
| Other | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Professor > Associate Professor | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 5 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 35% |
| Agricultural and Biological Sciences | 3 | 15% |
| Chemistry | 2 | 10% |
| Business, Management and Accounting | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 30% |