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Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds

Overview of attention for article published in BMC Research Notes, March 2013
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Title
Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds
Published in
BMC Research Notes, March 2013
DOI 10.1186/1756-0500-6-125
Pubmed ID
Authors

Raja Rajakani, Lokesh Narnoliya, Neelam Singh Sangwan, Rajender Singh Sangwan, Vikrant Gupta

Abstract

High quality RNA is a primary requisite for numerous molecular biological applications but is difficult to isolate from several plants rich in polysaccharides, polyphenolics and other secondary metabolites. These compounds either bind with nucleic acids or often co-precipitate at the final step and many times cannot be removed by conventional methods and kits. Addition of vinyl-pyrollidone polymers in extraction buffer efficiently removes polyphenolics to some extent, but, it failed in case of Azadirachta indica and several other medicinal and aromatic plants.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 2 2%
Malaysia 1 1%
United States 1 1%
France 1 1%
Unknown 80 94%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 20%
Student > Ph. D. Student 12 14%
Student > Master 10 12%
Student > Postgraduate 9 11%
Student > Bachelor 6 7%
Other 13 15%
Unknown 18 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 33 39%
Biochemistry, Genetics and Molecular Biology 15 18%
Pharmacology, Toxicology and Pharmaceutical Science 4 5%
Chemistry 4 5%
Medicine and Dentistry 3 4%
Other 6 7%
Unknown 20 24%