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Mendeley readers
Title |
Activated charcoal-mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds
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Published in |
BMC Research Notes, March 2013
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DOI | 10.1186/1756-0500-6-125 |
Pubmed ID | |
Authors |
Raja Rajakani, Lokesh Narnoliya, Neelam Singh Sangwan, Rajender Singh Sangwan, Vikrant Gupta |
Abstract |
High quality RNA is a primary requisite for numerous molecular biological applications but is difficult to isolate from several plants rich in polysaccharides, polyphenolics and other secondary metabolites. These compounds either bind with nucleic acids or often co-precipitate at the final step and many times cannot be removed by conventional methods and kits. Addition of vinyl-pyrollidone polymers in extraction buffer efficiently removes polyphenolics to some extent, but, it failed in case of Azadirachta indica and several other medicinal and aromatic plants. |
Mendeley readers
The data shown below were compiled from readership statistics for 85 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 2 | 2% |
Malaysia | 1 | 1% |
United States | 1 | 1% |
France | 1 | 1% |
Unknown | 80 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 17 | 20% |
Student > Ph. D. Student | 12 | 14% |
Student > Master | 10 | 12% |
Student > Postgraduate | 9 | 11% |
Student > Bachelor | 6 | 7% |
Other | 13 | 15% |
Unknown | 18 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 33 | 39% |
Biochemistry, Genetics and Molecular Biology | 15 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 5% |
Chemistry | 4 | 5% |
Medicine and Dentistry | 3 | 4% |
Other | 6 | 7% |
Unknown | 20 | 24% |