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Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells

Overview of attention for article published in Journal of Biomedical Science, November 2016
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Title
Defective autophagy leads to the suppression of stem-like features of CD271+ osteosarcoma cells
Published in
Journal of Biomedical Science, November 2016
DOI 10.1186/s12929-016-0297-5
Pubmed ID
Authors

Dong Zhang, Dong Zhang, Qing Zhao, Hao Sun, Lijuan Yin, Jiajun Wu, Jun Xu, Tianxiang He, Chunlei Yang, Chengwei Liang

Abstract

As an important stress-response mechanism, autophagy plays crucial role in the tumor formation and drug resistance of cancer cells including osteosarcoma (OS). OS cancer stem cells (CSCs) also are considered a key factor of tumorigenesis, drug resistance and tumor recurrence. However, the relationship between autophagy and OS CSCs still remains unclear. CD271+ OS CSCs and CD271- OS cells were isolated by magnetic activated cell sorting. The autophagy level was evaluated by the mRNA expression of autophagy genes, the protein level of LC3II and p62, and the mean number of GFP-LC3 dot per cell. Lentivirus-delivered specific shRNA was utilized to inhibit the corresponding gene expression. The cell viability was examined with CCK8 assay. The cell proliferation level was detected with BrdU staining assay. Cell death was determined by Annexin V/PI double staining of fluorescence activated cell sorting, lactate dehydrogenase release and caspase-3 activity. Tumorigenicity ability was evaluated by colony and sphere formation assay, the protein expression of stemness markers and tumor formation in nude mice. Our data indicated that CD271+ OS CSCs had a similar basic autophagy level with CD271- OS cells. Autophagy deficiency had no observable effects on the levels of cell proliferation and death both in CD271+ and CD271- OS cells under normal condition. However, CD271+ OS cells showed a higher autophagy activity than CD271- OS cells under hypoxia and low nutrient (LH) condition. Moreover, autophagy-deficient CD271+ OS cells lost the advantage of tolerance to LH condition compared to CD271- OS cells. Meanwhile, autophagy deficiency enhanced the sensitivity to chemotherapeutics in the CD271+ cells to the comparable level in the CD271- cells. More importantly, deficient-autophagy decreased the protein expression of stemness markers and caused the disappearance of the superiority in tumorigenicity in vitro and vivo in CD271+ OS cells. The results above demonstrated that autophagy contributes to the stem-like features of CD271+ OS CSCs. Inhibition of autophagy is a promising strategy in the CSCs-targeting OS therapy.

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 31%
Student > Bachelor 3 19%
Student > Ph. D. Student 2 13%
Unspecified 1 6%
Lecturer > Senior Lecturer 1 6%
Other 2 13%
Unknown 2 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 50%
Agricultural and Biological Sciences 2 13%
Unspecified 1 6%
Chemistry 1 6%
Medicine and Dentistry 1 6%
Other 0 0%
Unknown 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 November 2016.
All research outputs
#7,535,134
of 8,690,011 outputs
Outputs from Journal of Biomedical Science
#311
of 383 outputs
Outputs of similar age
#241,448
of 298,709 outputs
Outputs of similar age from Journal of Biomedical Science
#13
of 15 outputs
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