Title |
Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil
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Published in |
BMC Veterinary Research, April 2013
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DOI | 10.1186/1746-6148-9-85 |
Pubmed ID | |
Authors |
Marília Masello Junqueira Franco, Antonio Carlos Paes, Márcio Garcia Ribeiro, José Carlos de Figueiredo Pantoja, Adolfo Carlos Barreto Santos, Marcelo Miyata, Clarice Queico Fujimura Leite, Rodrigo Garcia Motta, Fernando José Paganini Listoni |
Abstract |
Mycobacterium spp. is one of the most important species of zoonotic pathogens that can be transmitted from cattle to humans. The presence of these opportunistic, pathogenic bacteria in bovine milk has emerged as a public-health concern, especially among individuals who consume raw milk and related dairy products. To address this concern, the Brazilian control and eradication program focusing on bovine tuberculosis, was established in 2001. However, bovine tuberculosis continues to afflict approximately 1,3 percent of the cattle in Brazil. In the present study, 300 samples of milk from bovine herds, obtained from both individual and collective bulk tanks and informal points of sale, were cultured on Löwenstein-Jensen and Stonebrink media. Polymerase chain reaction (PCR)-based tests and restriction-enzyme pattern analysis were then performed on the colonies exhibiting phenotypes suggestive of Mycobacterium spp., which were characterized as acid-fast bacilli. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 3 | 3% |
Colombia | 1 | <1% |
United Kingdom | 1 | <1% |
Denmark | 1 | <1% |
United States | 1 | <1% |
Unknown | 113 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 19 | 16% |
Student > Ph. D. Student | 18 | 15% |
Researcher | 11 | 9% |
Student > Bachelor | 11 | 9% |
Student > Postgraduate | 9 | 8% |
Other | 27 | 23% |
Unknown | 25 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 30 | 25% |
Agricultural and Biological Sciences | 30 | 25% |
Immunology and Microbiology | 11 | 9% |
Medicine and Dentistry | 11 | 9% |
Biochemistry, Genetics and Molecular Biology | 7 | 6% |
Other | 9 | 8% |
Unknown | 22 | 18% |