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The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique

Overview of attention for article published in BMC Microbiology, July 2009
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Mentioned by

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1 tweeter

Citations

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67 Dimensions

Readers on

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234 Mendeley
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1 CiteULike
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Title
The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique
Published in
BMC Microbiology, July 2009
DOI 10.1186/1471-2180-9-148
Pubmed ID
Authors

Sílvio B Santos, Carla M Carvalho, Sanna Sillankorva, Ana Nicolau, Eugénio C Ferreira, Joana Azeredo

Abstract

The Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages and to isolate mutants and new phages. Many phages form large and well-defined plaques that are easily observed so that they can be enumerated when plated by the DLA technique. However, some give rise to small and turbid plaques that are very difficult to detect and count. To overcome these problems, some authors have suggested the use of dyes to improve the contrast between the plaques and the turbid host lawns. It has been reported that some antibiotics stimulate bacteria to produce phages, resulting in an increase in final titer. Thus, antibiotics might contribute to increasing plaque size in solid media.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 234 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
Portugal 2 <1%
Australia 2 <1%
Nepal 1 <1%
Germany 1 <1%
Ireland 1 <1%
Unknown 224 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 45 19%
Student > Ph. D. Student 42 18%
Researcher 39 17%
Student > Bachelor 37 16%
Student > Doctoral Student 12 5%
Other 32 14%
Unknown 27 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 83 35%
Immunology and Microbiology 40 17%
Biochemistry, Genetics and Molecular Biology 36 15%
Veterinary Science and Veterinary Medicine 7 3%
Engineering 7 3%
Other 26 11%
Unknown 35 15%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2013.
All research outputs
#2,246,103
of 4,015,886 outputs
Outputs from BMC Microbiology
#419
of 799 outputs
Outputs of similar age
#44,698
of 85,946 outputs
Outputs of similar age from BMC Microbiology
#26
of 40 outputs
Altmetric has tracked 4,015,886 research outputs across all sources so far. This one is in the 25th percentile – i.e., 25% of other outputs scored the same or lower than it.
So far Altmetric has tracked 799 research outputs from this source. They receive a mean Attention Score of 2.8. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 85,946 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 29th percentile – i.e., 29% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.