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The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique

Overview of attention for article published in BMC Microbiology, July 2009
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1 X user

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Title
The use of antibiotics to improve phage detection and enumeration by the double-layer agar technique
Published in
BMC Microbiology, July 2009
DOI 10.1186/1471-2180-9-148
Pubmed ID
Authors

Sílvio B Santos, Carla M Carvalho, Sanna Sillankorva, Ana Nicolau, Eugénio C Ferreira, Joana Azeredo

Abstract

The Double-Layer Agar (DLA) technique is extensively used in phage research to enumerate and identify phages and to isolate mutants and new phages. Many phages form large and well-defined plaques that are easily observed so that they can be enumerated when plated by the DLA technique. However, some give rise to small and turbid plaques that are very difficult to detect and count. To overcome these problems, some authors have suggested the use of dyes to improve the contrast between the plaques and the turbid host lawns. It has been reported that some antibiotics stimulate bacteria to produce phages, resulting in an increase in final titer. Thus, antibiotics might contribute to increasing plaque size in solid media.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 276 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 1%
Portugal 2 <1%
Australia 2 <1%
Nepal 1 <1%
Germany 1 <1%
Ireland 1 <1%
Unknown 266 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 48 17%
Student > Master 48 17%
Student > Bachelor 43 16%
Researcher 43 16%
Student > Doctoral Student 13 5%
Other 33 12%
Unknown 48 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 86 31%
Biochemistry, Genetics and Molecular Biology 46 17%
Immunology and Microbiology 42 15%
Engineering 8 3%
Veterinary Science and Veterinary Medicine 7 3%
Other 29 11%
Unknown 58 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2013.
All research outputs
#15,270,698
of 22,708,120 outputs
Outputs from BMC Microbiology
#1,756
of 3,171 outputs
Outputs of similar age
#93,614
of 110,430 outputs
Outputs of similar age from BMC Microbiology
#8
of 10 outputs
Altmetric has tracked 22,708,120 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,171 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 110,430 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.