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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Nitric oxide has contrasting age-dependent effects on the functionality of murine hematopoietic stem cells
|
|---|---|
| Published in |
Stem Cell Research & Therapy, November 2016
|
| DOI | 10.1186/s13287-016-0433-x |
| Pubmed ID | |
| Authors |
Sapana Jalnapurkar, Shweta Singh, Moirangthem Ranjita Devi, Lalita Limaye, Vaijayanti Kale |
| Abstract |
The success of hematopoietic stem cell (HSC) transplantation is dependent on the quality of the donor HSCs. Some sources of HSCs display reduced engraftment efficiency either because of inadequate number (e.g., fetal liver and cord blood), or age-related dysfunction (e.g. in older individuals). Therefore, use of pharmacological compounds to improve functionality of HSCs is a forefront research area in hematology. Lineage negative (Lin(-)) cells isolated from murine bone marrow or sort-purified Lin(-)Sca-1(+)c-Kit(+)CD34(-) (LSK-CD34(-)) were treated with a nitric oxide donor, sodium nitroprusside (SNP). The cells were subjected to various phenotypic and functional assays. We found that SNP treatment of Lin(-) cells leads to an increase in the numbers of LSK-CD34(+) cells in them. Using sort-purified LSK CD34(-) HSCs, we show that this is related to acquisition of CD34 expression by LSK-CD34(-) cells, rather than proliferation of LSK-CD34(+) cells. Most importantly, this upregulated expression of CD34 had age-dependent contrasting effects on HSC functionality. Increased CD34 expression significantly improved the engraftment of juvenile HSCs (6-8 weeks); in sharp contrast, it reduced the engraftment of adult HSCs (10-12 weeks). The molecular mechanism behind this phenomenon involved nitric oxide (NO)-mediated differential induction of various transcription factors involved in commitment with regard to self-renewal in adult and juvenile HSCs, respectively. Preliminary experiments performed on cord blood-derived and mobilized peripheral blood-derived cells revealed that NO exerts age-dependent contrasting effects on human HSCs as well. This study demonstrates novel age-dependent contrasting effects of NO on HSC functionality and suggests that HSC age may be an important parameter in screening of various compounds for their use in manipulation of HSCs. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 13% |
| Student > Ph. D. Student | 2 | 13% |
| Student > Bachelor | 2 | 13% |
| Unspecified | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 5 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 33% |
| Chemistry | 2 | 13% |
| Unspecified | 1 | 7% |
| Nursing and Health Professions | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 5 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 November 2016.
All research outputs
#20,355,479
of 22,903,988 outputs
Outputs from Stem Cell Research & Therapy
#2,051
of 2,426 outputs
Outputs of similar age
#348,883
of 415,139 outputs
Outputs of similar age from Stem Cell Research & Therapy
#23
of 30 outputs
Altmetric has tracked 22,903,988 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,426 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 415,139 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.