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HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205

Overview of attention for article published in Epigenetics & Chromatin, December 2016
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  • Above-average Attention Score compared to outputs of the same age (57th percentile)

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4 tweeters

Citations

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36 Dimensions

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45 Mendeley
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Title
HOXA repression is mediated by nucleoporin Nup93 assisted by its interactors Nup188 and Nup205
Published in
Epigenetics & Chromatin, December 2016
DOI 10.1186/s13072-016-0106-0
Pubmed ID
Authors

Ajay S. Labade, Krishanpal Karmodiya, Kundan Sengupta

Abstract

The nuclear pore complex (NPC) mediates nuclear transport of RNA and proteins into and out of the nucleus. Certain nucleoporins have additional functions in chromatin organization and transcription regulation. Nup93 is a scaffold nucleoporin at the nuclear pore complex which is associated with human chromosomes 5, 7 and 16 and with the promoters of the HOXA gene as revealed by ChIP-on-chip studies using tiling microarrays for these chromosomes. However, the functional consequences of the association of Nup93 with HOXA is unknown. Here, we examined the association of Nup93 with the HOXA gene cluster and its consequences on HOXA gene expression in diploid colorectal cancer cells (DLD1). Nup93 showed a specific enrichment ~1 Kb upstream of the transcription start site of each of the HOXA1, HOXA3 and HOXA5 promoters, respectively. Furthermore, the association of Nup93 with HOXA was assisted by its interacting partners Nup188 and Nup205. The depletion of the Nup93 sub-complex significantly upregulated HOXA gene expression levels. However, expression levels of a control gene locus (GLCCI1) on human chromosome 7 were unaffected. Three-dimensional fluorescence in situ hybridization (3D-FISH) analyses revealed that the depletion of the Nup93 sub-complex (but not Nup98) disengages the HOXA gene locus from the nuclear periphery, suggesting a potential role for Nup93 in tethering and repressing the HOXA gene cluster. Consistently, Nup93 knockdown increased active histone marks (H3K9ac), decreased repressive histone marks (H3K27me3) on the HOXA1 promoter and increased transcription elongation marks (H3K36me3) within the HOXA1 gene. Moreover, the combined depletion of Nup93 and CTCF (a known organizer of HOXA gene cluster) but not Nup93 alone, significantly increased GLCCI1 gene expression levels. Taken together, this suggests a novel role for Nup93 and its interactors in repressing the HOXA gene cluster. This study reveals that the nucleoporin Nup93 assisted by its interactors Nup188 and Nup205 mediates the repression of HOXA gene expression.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Austria 2 4%
Unknown 43 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 27%
Researcher 6 13%
Student > Doctoral Student 6 13%
Student > Bachelor 6 13%
Student > Postgraduate 3 7%
Other 3 7%
Unknown 9 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 47%
Agricultural and Biological Sciences 10 22%
Medicine and Dentistry 2 4%
Immunology and Microbiology 1 2%
Engineering 1 2%
Other 0 0%
Unknown 10 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 May 2019.
All research outputs
#8,169,738
of 15,134,662 outputs
Outputs from Epigenetics & Chromatin
#287
of 444 outputs
Outputs of similar age
#159,910
of 384,926 outputs
Outputs of similar age from Epigenetics & Chromatin
#53
of 58 outputs
Altmetric has tracked 15,134,662 research outputs across all sources so far. This one is in the 45th percentile – i.e., 45% of other outputs scored the same or lower than it.
So far Altmetric has tracked 444 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 384,926 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 6th percentile – i.e., 6% of its contemporaries scored the same or lower than it.