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X Demographics
Mendeley readers
Attention Score in Context
| Title |
An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies
|
|---|---|
| Published in |
Parasites & Vectors, December 2016
|
| DOI | 10.1186/s13071-016-1913-7 |
| Pubmed ID | |
| Authors |
Ole Lagatie, Michelle Merino, Linda Batsa Debrah, Alexander Y. Debrah, Lieven J. Stuyver |
| Abstract |
Diagnostic procedures for the diagnosis of infection with the nematode parasite Onchocerca volvulus are currently based on the microscopic detection of microfilariae in skin biopsies. Alternative approaches based on amplification of parasitic DNA in these skin biopsies are currently being explored. Mostly this is based on the detection of the O-150 repeat sequence using PCR based techniques. An isothermal, loop-mediated amplification method has been designed using the mitochondrial O. volvulus cox1 gene as a target. Analysis of dilution series of synthetic DNA containing the targeted sequence show a non-linear dose-response curve, as is usually the case for isothermal amplification methods. Evaluation of cross-reactivity with the heterologous sequence from the closely related parasites Wuchereria bancrofti, Loa loa and Brugia malayi demonstrated strong specificity, as none of these sequences was amplified. The assay however amplified both O. volvulus and O. ochengi DNA, but with a different melting point that can be used to discriminate between the species. Evaluation of this assay in a set of skin snip biopsies collected in an endemic area in Ghana showed a high correlation with O-150 qPCR and also demonstrated a similar sensitivity. Compared to qPCR, LAMP had a sensitivity of 88.2% and a specificity of 99.2%. We have developed a sensitive and specific loop-mediated amplification method for detection of O. volvulus DNA in skin biopsies that is capable of providing results within 30 min. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 69 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 17% |
| Student > Ph. D. Student | 10 | 14% |
| Professor > Associate Professor | 4 | 6% |
| Student > Master | 4 | 6% |
| Student > Bachelor | 3 | 4% |
| Other | 10 | 14% |
| Unknown | 26 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 12% |
| Immunology and Microbiology | 8 | 12% |
| Biochemistry, Genetics and Molecular Biology | 7 | 10% |
| Unspecified | 4 | 6% |
| Nursing and Health Professions | 3 | 4% |
| Other | 10 | 14% |
| Unknown | 29 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2016.
All research outputs
#17,832,285
of 22,908,162 outputs
Outputs from Parasites & Vectors
#3,833
of 5,479 outputs
Outputs of similar age
#287,753
of 416,461 outputs
Outputs of similar age from Parasites & Vectors
#53
of 84 outputs
Altmetric has tracked 22,908,162 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,479 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 416,461 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.