Title |
IL-21 promotes the expansion of CD27+CD28+ tumor infiltrating lymphocytes with high cytotoxic potential and low collateral expansion of regulatory T cells
|
---|---|
Published in |
Journal of Translational Medicine, February 2013
|
DOI | 10.1186/1479-5876-11-37 |
Pubmed ID | |
Authors |
Saskia JAM Santegoets, Annelies W Turksma, Megan M Suhoski, Anita GM Stam, Steve M Albelda, Erik Hooijberg, Rik J Scheper, Alfons JM van den Eertwegh, Winald R Gerritsen, Daniel J Powell, Carl H June, Tanja D de Gruijl |
Abstract |
Adoptive cell transfer of tumor infiltrating lymphocytes has shown clinical efficacy in the treatment of melanoma and is now also being explored in other tumor types. Generation of sufficient numbers of effector T cells requires extensive ex vivo expansion, often at the cost of T cell differentiation and potency. For the past 20 years, IL-2 has been the key cytokine applied in the expansion of TIL for ACT. However, the use of IL-2 has also led to collateral expansion of regulatory T cells (Tregs) and progressive T cell differentiation, factors known to limit in vivo persistence and activity of transferred TIL. The use of alternative T cell growth factors is therefore warranted. Here, we have compared the effects of IL-2, -15 and -21 cytokines on the expansion and activation of TIL from single-cell suspensions of non-small cell lung cancer, ovarian cancer and melanoma. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 20% |
United States | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 60% |
Members of the public | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 2% |
Netherlands | 1 | <1% |
Unknown | 100 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 24 | 23% |
Researcher | 23 | 22% |
Student > Master | 14 | 14% |
Student > Bachelor | 9 | 9% |
Professor | 8 | 8% |
Other | 9 | 9% |
Unknown | 16 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 29 | 28% |
Medicine and Dentistry | 19 | 18% |
Immunology and Microbiology | 19 | 18% |
Biochemistry, Genetics and Molecular Biology | 9 | 9% |
Engineering | 3 | 3% |
Other | 6 | 6% |
Unknown | 18 | 17% |