Title |
IMP: a pipeline for reproducible reference-independent integrated metagenomic and metatranscriptomic analyses
|
---|---|
Published in |
Genome Biology, December 2016
|
DOI | 10.1186/s13059-016-1116-8 |
Pubmed ID | |
Authors |
Shaman Narayanasamy, Yohan Jarosz, Emilie E. L. Muller, Anna Heintz-Buschart, Malte Herold, Anne Kaysen, Cédric C. Laczny, Nicolás Pinel, Patrick May, Paul Wilmes |
Abstract |
Existing workflows for the analysis of multi-omic microbiome datasets are lab-specific and often result in sub-optimal data usage. Here we present IMP, a reproducible and modular pipeline for the integrated and reference-independent analysis of coupled metagenomic and metatranscriptomic data. IMP incorporates robust read preprocessing, iterative co-assembly, analyses of microbial community structure and function, automated binning, as well as genomic signature-based visualizations. The IMP-based data integration strategy enhances data usage, output volume, and output quality as demonstrated using relevant use-cases. Finally, IMP is encapsulated within a user-friendly implementation using Python and Docker. IMP is available at http://r3lab.uni.lu/web/imp/ (MIT license). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 5 | 14% |
Luxembourg | 4 | 11% |
United States | 4 | 11% |
Netherlands | 2 | 5% |
France | 2 | 5% |
Canada | 1 | 3% |
Switzerland | 1 | 3% |
Ireland | 1 | 3% |
Norway | 1 | 3% |
Other | 4 | 11% |
Unknown | 12 | 32% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 21 | 57% |
Members of the public | 14 | 38% |
Science communicators (journalists, bloggers, editors) | 2 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 2 | <1% |
Indonesia | 1 | <1% |
France | 1 | <1% |
Norway | 1 | <1% |
Finland | 1 | <1% |
United Kingdom | 1 | <1% |
United States | 1 | <1% |
Unknown | 275 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 64 | 23% |
Researcher | 57 | 20% |
Student > Master | 42 | 15% |
Student > Bachelor | 20 | 7% |
Student > Postgraduate | 15 | 5% |
Other | 36 | 13% |
Unknown | 49 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 73 | 26% |
Biochemistry, Genetics and Molecular Biology | 63 | 22% |
Environmental Science | 20 | 7% |
Immunology and Microbiology | 18 | 6% |
Computer Science | 14 | 5% |
Other | 34 | 12% |
Unknown | 61 | 22% |