Title |
Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia
|
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Published in |
Virology Journal, May 2013
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DOI | 10.1186/1743-422x-10-150 |
Pubmed ID | |
Authors |
Awatef El Moussi, Mohamed Ali Ben Hadj Kacem, Francisco Pozo, Juan Ledesma, Maria Teresa Cuevas, Inmaculada Casas, Amine Slim |
Abstract |
We present major results concerning isolation and determination of the nucleotide sequence of hemagglutinin (HA1) of the pandemic (H1N1)pdm09 influenza viruses found in Tunisia. Amino acid analysis revealed minor amino acid changes in the antigenic or receptor-binding domains. We found mutations that were also present in 1918 pandemic virus, which includes S183P in 4 and S185T mutation in 19 of 27 viruses analyzed from 2011, while none of the 2009 viruses carried these mutations. Also two specific amino acid differences into N-glycosylation sites (N288T and N276H) were detected. The phylogenetic analysis revealed that the majority of the Tunisian isolates clustered with clade A/St. Petersburg/27/2011 viruses characterized by D97N and S185T mutations. However it also reveals a trend of 2010 strains to accumulate amino acid variation and form new phylogenetic clade with three specific amino acid substitutions: V47I, E172K and K308E. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 7 | 26% |
Student > Ph. D. Student | 4 | 15% |
Professor | 3 | 11% |
Student > Bachelor | 3 | 11% |
Other | 2 | 7% |
Other | 4 | 15% |
Unknown | 4 | 15% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 5 | 19% |
Medicine and Dentistry | 5 | 19% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Immunology and Microbiology | 4 | 15% |
Veterinary Science and Veterinary Medicine | 1 | 4% |
Other | 3 | 11% |
Unknown | 5 | 19% |