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Attention Score in Context
| Title |
Instability of standard PCR reference genes in adipose-derived stem cells during propagation, differentiation and hypoxic exposure
|
|---|---|
| Published in |
BMC Molecular and Cell Biology, October 2008
|
| DOI | 10.1186/1471-2199-9-98 |
| Pubmed ID | |
| Authors |
Trine Fink, Pia Lund, Linda Pilgaard, Jeppe Grøndahl Rasmussen, Meg Duroux, Vladimir Zachar |
| Abstract |
For the accurate determination of gene expression changes during growth and differentiation studies on adipose-derived stem cells (ASCs), quantitative real-time RT-PCR has become a method of choice. The technology is very sensitive, however, without a proper selection of reference genes, to which the genes of interest are normalized, erroneous results may be obtained. In this study, we have compared the gene expression levels of a panel of twelve widely used reference genes during hypoxic culture, osteogenic and chondrogenic differentiation, and passaging of primary human ASCs. We found that several of the commonly used reference genes including 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and beta-actin were unsuitable for normalization in the conditions we tested, whereas tyrosine 3/tryptophan 5-monooxygenase activation protein (YMHAZ), TATAA-box binding protein (TBP), beta-glucuronidase (GUSB) were the most stable across all conditions. When determining gene expression levels in adipose-derived stem cells, we recommend normalizing transcription levels to the geometric mean of YMHAZ, TBP and GUSB. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 158 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 1% |
| United Kingdom | 2 | 1% |
| France | 1 | <1% |
| Brazil | 1 | <1% |
| Switzerland | 1 | <1% |
| India | 1 | <1% |
| Greece | 1 | <1% |
| Denmark | 1 | <1% |
| Unknown | 148 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 46 | 29% |
| Student > Bachelor | 29 | 18% |
| Student > Master | 23 | 15% |
| Researcher | 15 | 9% |
| Professor > Associate Professor | 11 | 7% |
| Other | 22 | 14% |
| Unknown | 12 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 66 | 42% |
| Medicine and Dentistry | 33 | 21% |
| Biochemistry, Genetics and Molecular Biology | 19 | 12% |
| Engineering | 8 | 5% |
| Immunology and Microbiology | 3 | 2% |
| Other | 8 | 5% |
| Unknown | 21 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 December 2016.
All research outputs
#20,656,161
of 25,374,647 outputs
Outputs from BMC Molecular and Cell Biology
#935
of 1,233 outputs
Outputs of similar age
#96,708
of 104,769 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#25
of 27 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.