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Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations

Overview of attention for article published in BMC Ecology and Evolution, May 2013
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Title
Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations
Published in
BMC Ecology and Evolution, May 2013
DOI 10.1186/1472-6785-13-21
Pubmed ID
Authors

Stefan Koenigstein, Kevin Pöhlmann, Christoph Held, Doris Abele

Abstract

Rising temperatures and other environmental factors influenced by global climate change can cause increased physiological stress for many species and lead to range shifts or regional population extinctions. To advance the understanding of species' response to change and establish links between individual and ecosystem adaptations, physiological reactions have to be compared between populations living in different environments. Although changes in expression of stress genes are relatively easy to quantify, methods for reliable comparison of the data remain a contentious issue. Using normalization algorithms and further methodological considerations, we compare cellular stress response gene expression levels measured by RT-qPCR after air exposure experiments among different subpopulations of three species of the intertidal limpet Nacella.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 4%
Unknown 45 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 30%
Researcher 12 26%
Student > Master 3 6%
Student > Doctoral Student 2 4%
Student > Bachelor 2 4%
Other 6 13%
Unknown 8 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 49%
Biochemistry, Genetics and Molecular Biology 4 9%
Environmental Science 3 6%
Psychology 2 4%
Medicine and Dentistry 2 4%
Other 2 4%
Unknown 11 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 May 2013.
All research outputs
#17,285,668
of 25,373,627 outputs
Outputs from BMC Ecology and Evolution
#2,928
of 3,714 outputs
Outputs of similar age
#131,491
of 207,268 outputs
Outputs of similar age from BMC Ecology and Evolution
#42
of 57 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,714 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.5. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 207,268 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.