Title |
Biodistribution of biodegradable polymeric nano-carriers loaded with busulphan and designed for multimodal imaging
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Published in |
Journal of Nanobiotechnology, December 2016
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DOI | 10.1186/s12951-016-0239-0 |
Pubmed ID | |
Authors |
Heba Asem, Ying Zhao, Fei Ye, Åsa Barrefelt, Manuchehr Abedi-Valugerdi, Ramy El-Sayed, Ibrahim El-Serafi, Khalid M. Abu-Salah, Jörg Hamm, Mamoun Muhammed, Moustapha Hassan |
Abstract |
Multifunctional nanocarriers for controlled drug delivery, imaging of disease development and follow-up of treatment efficacy are promising novel tools for disease diagnosis and treatment. In the current investigation, we present a multifunctional theranostic nanocarrier system for anticancer drug delivery and molecular imaging. Superparamagnetic iron oxide nanoparticles (SPIONs) as an MRI contrast agent and busulphan as a model for lipophilic antineoplastic drugs were encapsulated into poly (ethylene glycol)-co-poly (caprolactone) (PEG-PCL) micelles via the emulsion-evaporation method, and PEG-PCL was labelled with VivoTag 680XL fluorochrome for in vivo fluorescence imaging. Busulphan entrapment efficiency was 83% while the drug release showed a sustained pattern over 10 h. SPION loaded-PEG-PCL micelles showed contrast enhancement in T 2 *-weighted MRI with high r 2* relaxivity. In vitro cellular uptake of PEG-PCL micelles labeled with fluorescein in J774A cells was found to be time-dependent. The maximum uptake was observed after 24 h of incubation. The biodistribution of PEG-PCL micelles functionalized with VivoTag 680XL was investigated in Balb/c mice over 48 h using in vivo fluorescence imaging. The results of real-time live imaging were then confirmed by ex vivo organ imaging and histological examination. Generally, PEG-PCL micelles were highly distributed into the lungs during the first 4 h post intravenous administration, then redistributed and accumulated in liver and spleen until 48 h post administration. No pathological impairment was found in the major organs studied. Thus, with loaded contrast agent and conjugated fluorochrome, PEG-PCL micelles as biodegradable and biocompatible nanocarriers are efficient multimodal imaging agents, offering high drug loading capacity, and sustained drug release. These might offer high treatment efficacy and real-time tracking of the drug delivery system in vivo, which is crucial for designing of an efficient drug delivery system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 48 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 23% |
Student > Ph. D. Student | 11 | 23% |
Other | 5 | 10% |
Student > Bachelor | 4 | 8% |
Student > Doctoral Student | 2 | 4% |
Other | 4 | 8% |
Unknown | 11 | 23% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 15% |
Medicine and Dentistry | 6 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 10% |
Chemistry | 4 | 8% |
Psychology | 3 | 6% |
Other | 8 | 17% |
Unknown | 15 | 31% |