Title |
Identification of differentially expressed genes associated with semigamy in Pima cotton (Gossypium barbadenseL.) through comparative microarray analysis
|
---|---|
Published in |
BMC Plant Biology, March 2011
|
DOI | 10.1186/1471-2229-11-49 |
Pubmed ID | |
Authors |
Jessica Curtiss, Laura Rodriguez-Uribe, J McD Stewart, Jinfa Zhang |
Abstract |
Semigamy in cotton is a type of facultative apomixis controlled by an incompletely dominant autosomal gene (Se). During semigamy, the sperm and egg cells undergo cellular fusion, but the sperm and egg nucleus fail to fuse in the embryo sac, giving rise to diploid, haploid, or chimeric embryos composed of sectors of paternal and maternal origin. In this study we sought to identify differentially expressed genes related to the semigamy genotype by implementing a comparative microarray analysis of anthers and ovules between a non-semigametic Pima S-1 cotton and its doubled haploid natural isogenic mutant semigametic 57-4. Selected differentially expressed genes identified by the microarray results were then confirmed using quantitative reverse transcription PCR (qRT-PCR). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 24% |
Researcher | 3 | 10% |
Student > Postgraduate | 3 | 10% |
Professor | 2 | 7% |
Professor > Associate Professor | 2 | 7% |
Other | 5 | 17% |
Unknown | 7 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 19 | 66% |
Sports and Recreations | 1 | 3% |
Unknown | 9 | 31% |