Title |
Lentivirus-mediated transgene delivery to the hippocampus reveals sub-field specific differences in expression
|
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Published in |
BMC Neuroscience, January 2009
|
DOI | 10.1186/1471-2202-10-2 |
Pubmed ID | |
Authors |
Lenneke WA van Hooijdonk, Muhammad Ichwan, Thomas F Dijkmans, Theo G Schouten, Marijke WA de Backer, Roger AH Adan, Fons J Verbeek, Erno Vreugdenhil, Carlos P Fitzsimons |
Abstract |
In the adult hippocampus, the granule cell layer of the dentate gyrus is a heterogeneous structure formed by neurons of different ages, morphologies and electrophysiological properties. Retroviral vectors have been extensively used to transduce cells of the granule cell layer and study their inherent properties in an intact brain environment. In addition, lentivirus-based vectors have been used to deliver transgenes to replicative and non-replicative cells as well, such as post mitotic neurons of the CNS. However, only few studies have been dedicated to address the applicability of these widespread used vectors to hippocampal cells in vivo. Therefore, the aim of this study was to extensively characterize the cell types that are effectively transduced in vivo by VSVg-pseudotyped lentivirus-based vectors in the hippocampus dentate gyrus. |
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