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Mendeley readers
Attention Score in Context
| Title |
Combinatory optimization of chromosomal integrated mevalonate pathway for β-carotene production in Escherichia coli
|
|---|---|
| Published in |
Microbial Cell Factories, December 2016
|
| DOI | 10.1186/s12934-016-0607-3 |
| Pubmed ID | |
| Authors |
Lijun Ye, Chunzhi Zhang, Changhao Bi, Qingyan Li, Xueli Zhang |
| Abstract |
Plasmid expression is a popular method in studies of MVA pathway for isoprenoid production in Escherichia coli. However, heterologous gene expression with plasmid is often not stable and might burden growth of host cells, decreases cell mass and product yield. In this study, MVA pathway was divided into three modules, and two heterologous modules were integrated into the E. coli chromosome. These modules were individually modulated with regulatory parts to optimize efficiency of the pathway in terms of downstream isoprenoid production. MVA pathway modules Hmg1-erg12 operon and mvaS-mvaA-mavD1 operon were integrated into E. coli chromosome followed by modulation with promoters with varied strength. Along with activation of atoB, a 26% increase of β-carotene production with no effect on cell growth was obtained. With a combinatory modulation of two key enzymes mvas and Hmg1 with degenerate RBS library, β-carotene showed a further increase of 51%. Our study provides a novel strategy for improving production of a target compound through integration and modulation of heterologous pathways in both transcription and translation level. In addition, a genetically hard-coded chassis with both efficient MEP and MVA pathways for isoprenoid precursor supply was constructed in this work. |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 2% |
| France | 1 | 2% |
| Unknown | 59 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 26% |
| Researcher | 8 | 13% |
| Student > Master | 8 | 13% |
| Other | 4 | 7% |
| Student > Doctoral Student | 3 | 5% |
| Other | 8 | 13% |
| Unknown | 14 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 19 | 31% |
| Biochemistry, Genetics and Molecular Biology | 15 | 25% |
| Unspecified | 3 | 5% |
| Chemical Engineering | 3 | 5% |
| Chemistry | 3 | 5% |
| Other | 3 | 5% |
| Unknown | 15 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2017.
All research outputs
#2,149,326
of 22,925,760 outputs
Outputs from Microbial Cell Factories
#60
of 1,608 outputs
Outputs of similar age
#45,139
of 416,629 outputs
Outputs of similar age from Microbial Cell Factories
#1
of 32 outputs
Altmetric has tracked 22,925,760 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,608 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 416,629 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.