Title |
Routine performance and errors of 454 HLA exon sequencing in diagnostics
|
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Published in |
BMC Bioinformatics, June 2013
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DOI | 10.1186/1471-2105-14-176 |
Pubmed ID | |
Authors |
Norbert Niklas, Johannes Pröll, Martin Danzer, Stephanie Stabentheiner, Katja Hofer, Christian Gabriel |
Abstract |
Next-generation sequencing (NGS) has changed genomics significantly. More and more applications strive for sequencing with different platforms. Now, in 2012, after a decade of development and evolution, NGS has been accepted for a variety of research fields. Determination of sequencing errors is essential in order to follow next-generation sequencing beyond research use only. This study describes the overall 454 system performance of using multiple GS Junior runs with an in-house established and validated diagnostic assay for human leukocyte antigen (HLA) exon sequencing. Based on this data, we extracted, evaluated and characterized errors and variants of 60 HLA loci per run with respect to their adjacencies. |
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Norway | 2 | 20% |
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Germany | 1 | 10% |
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Demographic breakdown
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Members of the public | 2 | 20% |
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Mendeley readers
Geographical breakdown
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Sweden | 1 | 2% |
United Arab Emirates | 1 | 2% |
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Russia | 1 | 2% |
Unknown | 35 | 85% |
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Student > Bachelor | 6 | 15% |
Student > Doctoral Student | 4 | 10% |
Other | 4 | 10% |
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Unknown | 1 | 2% |
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Engineering | 3 | 7% |
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Unknown | 4 | 10% |