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Mendeley readers
Attention Score in Context
| Title |
Engineering Corynebacterium glutamicum for violacein hyper production
|
|---|---|
| Published in |
Microbial Cell Factories, August 2016
|
| DOI | 10.1186/s12934-016-0545-0 |
| Pubmed ID | |
| Authors |
Hongnian Sun, Dongdong Zhao, Bin Xiong, Chunzhi Zhang, Changhao Bi |
| Abstract |
Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from L-tryptophan. Corynebacterium glutamicum ATCC 21850 that could produce 162.98 mg L(-1) tryptophan was employed as a novel host for metabolic engineering chassis. Heterologous vio operon from Chromobacterium violaceum was over-expressed in ATCC 21850 strain with constitutive promoter to have obtained 532 mg L(-1) violacein. Considering toxicity of violacein, vio operon was expressed with inducible promoter and 629 mg L(-1) violacein was obtained in batch culture. Due to the economical coding nature of vio operon, the compressed RBS of vio genes were replaced with complete strong C. glutamicum ones. And extended expression units were assembled to form a synthetic operon. With this strategy, 1116 mg L(-1) violacein in batch culture was achieved. Fermentation process was then optimized by studying induction time, induction concentration, culture composition and fermentation temperature. as a result, a titer of 5436 mg L(-1) and a productivity of 47 mg L(-1) h(-1) were achieved in 3 L bioreactor. With metabolic engineering and fermentation optimization practice, C. glutamicum 21850 (pEC-C-vio1) was able to produce violacein with both titer and productivity at the highest level ever reported. Due to advantages of mature C. glutamicum fermentation industry, this work has built basis for commercial production of violacein. |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 1% |
| China | 1 | 1% |
| Germany | 1 | 1% |
| Unknown | 84 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 21 | 24% |
| Student > Bachelor | 13 | 15% |
| Student > Master | 10 | 11% |
| Researcher | 8 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 8 | 9% |
| Unknown | 23 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 28 | 32% |
| Agricultural and Biological Sciences | 20 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 3% |
| Chemical Engineering | 3 | 3% |
| Immunology and Microbiology | 2 | 2% |
| Other | 5 | 6% |
| Unknown | 26 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 January 2017.
All research outputs
#4,202,008
of 22,931,367 outputs
Outputs from Microbial Cell Factories
#227
of 1,608 outputs
Outputs of similar age
#71,988
of 341,663 outputs
Outputs of similar age from Microbial Cell Factories
#2
of 39 outputs
Altmetric has tracked 22,931,367 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,608 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 341,663 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 77% of its contemporaries.
We're also able to compare this research output to 39 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.