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Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass

Overview of attention for article published in Biotechnology for Biofuels, January 2017
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Title
Integrated omics analyses reveal the details of metabolic adaptation of Clostridium thermocellum to lignocellulose-derived growth inhibitors released during the deconstruction of switchgrass
Published in
Biotechnology for Biofuels, January 2017
DOI 10.1186/s13068-016-0697-5
Pubmed ID
Authors

Suresh Poudel, Richard J. Giannone, Miguel Rodriguez, Babu Raman, Madhavi Z. Martin, Nancy L. Engle, Jonathan R. Mielenz, Intawat Nookaew, Steven D. Brown, Timothy J. Tschaplinski, David Ussery, Robert L. Hettich

Abstract

Clostridium thermocellum is capable of solubilizing and converting lignocellulosic biomass into ethanol. Although much of the work-to-date has centered on characterizing this microbe's growth on model cellulosic substrates, such as cellobiose, Avicel, or filter paper, it is vitally important to understand its metabolism on more complex, lignocellulosic substrates to identify relevant industrial bottlenecks that could undermine efficient biofuel production. To this end, we have examined a time course progression of C. thermocellum grown on switchgrass to assess the metabolic and protein changes that occur during the conversion of plant biomass to ethanol. The most striking feature of the metabolome was the observed accumulation of long-chain, branched fatty acids over time, implying an adaptive restructuring of C. thermocellum's cellular membrane as the culture progresses. This is undoubtedly a response to the gradual accumulation of lignocellulose-derived inhibitory compounds as the organism deconstructs the switchgrass to access the embedded cellulose. Corroborating the metabolomics data, proteomic analysis revealed a corresponding time-dependent increase in various enzymes, including those involved in the interconversion of branched amino acids valine, leucine, and isoleucine to iso- and anteiso-fatty acid precursors. Additionally, the metabolic accumulation of hemicellulose-derived sugars and sugar alcohols concomitant with increased abundance of enzymes involved in C5 sugar metabolism/pentose phosphate pathway indicates that C. thermocellum shifts glycolytic intermediates to alternate pathways to modulate overall carbon flux in response to C5 sugar metabolites that increase during lignocellulose deconstruction. Integrated omic platforms provided complementary systems biological information that highlight C. thermocellum's specific response to cytotoxic inhibitors released during the deconstruction and utilization of switchgrass. These additional viewpoints allowed us to fully realize the level to which the organism adapts to an increasingly challenging culture environment-information that will prove critical to C. thermocellum's industrial efficacy.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 70 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 1%
Unknown 69 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 27%
Student > Master 10 14%
Researcher 10 14%
Student > Bachelor 7 10%
Student > Doctoral Student 2 3%
Other 8 11%
Unknown 14 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 36%
Biochemistry, Genetics and Molecular Biology 15 21%
Engineering 3 4%
Chemical Engineering 3 4%
Immunology and Microbiology 2 3%
Other 9 13%
Unknown 13 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 February 2017.
All research outputs
#4,644,053
of 9,048,564 outputs
Outputs from Biotechnology for Biofuels
#348
of 750 outputs
Outputs of similar age
#157,729
of 307,014 outputs
Outputs of similar age from Biotechnology for Biofuels
#17
of 43 outputs
Altmetric has tracked 9,048,564 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 750 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 307,014 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.