Fuling You, Qiao Li, Guifang Jin, Yaojie Zheng, Jingrong Chen, Hong Yang
Abstract
Deposition of aggregated amyloid beta (Aβ) protein is hallmark of Alzheimer's disease, leading to dysfunction and apoptosis of neurons. The isoflavone phytoestrogen compound genistein (Gen) exerts a significant protective effect against Aβ25-35 induced neurotoxicity and mitochondrial damage in rat pheochromocytoma (PC12) cells. However, the mechanisms underlying Gen's rescue remain elusive. Therefore we endeavored to research further the molecular mechanisms underlying Gen's inhibition of Aβ25-35 induced apoptosis of neurons.
We found that Gen dramatically suppressed the activation by Aβ25-35 of p-c-Jun N-terminal kinase (p-JNK), and also inhibited the JNK-dependent decreased of Bcl-w and increased of Bim. Furthermore, Gen significantly reduced the cytoplasmic concentrations of cytochrome c and Smac protein as well as caspase-3 activity. Additionally, pretreatment with JNK inhibitor SP600125 effectively suppressed Aβ25-35 induced PC12 cell cytotoxicity.
Taken together, the results suggested that Gen protects PC12 cells from Aβ25-35 induced neurotoxicity by interfering with p-JNK activation, thus attenuating the JNK-dependent apoptosis through the mitochondrial pathway. These findings constitute novel insights into the pathway for Aβ25-35 toxicity, and the neuroprotective action of Gen.
Pharmacology, Toxicology and Pharmaceutical Science
4
11%
Medicine and Dentistry
3
9%
Neuroscience
3
9%
Engineering
2
6%
Other
3
9%
Unknown
16
46%
Attention Score in Context
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 January 2017.
All research outputs
#3,217,757
of 22,931,367 outputs
Outputs from BMC Neuroscience
#134
of 1,249 outputs
Outputs of similar age
#68,332
of 422,128 outputs
Outputs of similar age from BMC Neuroscience
#6
of 33 outputs
Altmetric has tracked 22,931,367 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,249 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 88% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 422,128 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 83% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.
