Title |
A cost effective real-time PCR for the detection of adenovirus from viral swabs
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Published in |
Virology Journal, June 2013
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DOI | 10.1186/1743-422x-10-184 |
Pubmed ID | |
Authors |
Turkiya Al-Siyabi, Khalifa Binkhamis, Melanie Wilcox, Sallene Wong, Kanti Pabbaraju, Raymond Tellier, Todd F Hatchette, Jason J LeBlanc |
Abstract |
Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 40 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 18% |
Researcher | 6 | 15% |
Student > Ph. D. Student | 5 | 13% |
Student > Bachelor | 4 | 10% |
Student > Doctoral Student | 2 | 5% |
Other | 6 | 15% |
Unknown | 10 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 8 | 20% |
Biochemistry, Genetics and Molecular Biology | 8 | 20% |
Immunology and Microbiology | 5 | 13% |
Medicine and Dentistry | 4 | 10% |
Environmental Science | 1 | 3% |
Other | 5 | 13% |
Unknown | 9 | 23% |