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A cost effective real-time PCR for the detection of adenovirus from viral swabs

Overview of attention for article published in Virology Journal, June 2013
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (64th percentile)

Mentioned by

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1 X user
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1 patent

Citations

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8 Dimensions

Readers on

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40 Mendeley
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Title
A cost effective real-time PCR for the detection of adenovirus from viral swabs
Published in
Virology Journal, June 2013
DOI 10.1186/1743-422x-10-184
Pubmed ID
Authors

Turkiya Al-Siyabi, Khalifa Binkhamis, Melanie Wilcox, Sallene Wong, Kanti Pabbaraju, Raymond Tellier, Todd F Hatchette, Jason J LeBlanc

Abstract

Compared to traditional testing strategies, nucleic acid amplification tests such as real-time PCR offer many advantages for the detection of human adenoviruses. However, commercial assays are expensive and cost prohibitive for many clinical laboratories. To overcome fiscal challenges, a cost effective strategy was developed using a combination of homogenization and heat treatment with an "in-house" real-time PCR. In 196 swabs submitted for adenovirus detection, this crude extraction method showed performance characteristics equivalent to viral DNA obtained from a commercial nucleic acid extraction. In addition, the in-house real-time PCR outperformed traditional testing strategies using virus culture, with sensitivities of 100% and 69.2%, respectively. Overall, the combination of homogenization and heat treatment with a sensitive in-house real-time PCR provides accurate results at a cost comparable to viral culture.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 40 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 18%
Researcher 6 15%
Student > Ph. D. Student 5 13%
Student > Bachelor 4 10%
Student > Doctoral Student 2 5%
Other 6 15%
Unknown 10 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 20%
Biochemistry, Genetics and Molecular Biology 8 20%
Immunology and Microbiology 5 13%
Medicine and Dentistry 4 10%
Environmental Science 1 3%
Other 5 13%
Unknown 9 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 August 2021.
All research outputs
#7,932,336
of 25,366,663 outputs
Outputs from Virology Journal
#918
of 3,387 outputs
Outputs of similar age
#62,928
of 203,929 outputs
Outputs of similar age from Virology Journal
#32
of 105 outputs
Altmetric has tracked 25,366,663 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,387 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 24.6. This one has gotten more attention than average, scoring higher than 71% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,929 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 105 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.