The multi-kingdom microbiome of the goat gastrointestinal tract

Yanhong Cao, Tong Feng, Yingjian Wu, Yixue Xu, Li Du, Teng Wang, Yuhong Luo, Yan Wang, Zhipeng Li, Zeyi Xuan, Shaomei Chen, Na Yao, Na L. Gao, Qian Xiao, Kongwei Huang, Xiaobo Wang, Kuiqing Cui, Saif ur Rehman, Xiangfang Tang, Dewu Liu, Hongbing Han, Ying Li, Wei-Hua Chen, Qingyou Liu

Goat is an important livestock worldwide, which plays an indispensable role in human life by providing meat, milk, fiber, and pelts. Despite recent significant advances in microbiome studies, a comprehensive survey on the goat microbiomes covering gastrointestinal tract (GIT) sites, developmental stages, feeding styles, and geographical factors is still unavailable. Here, we surveyed its multi-kingdom microbial communities using 497 samples from ten sites along the goat GIT. We reconstructed a goat multi-kingdom microbiome catalog (GMMC) including 4004 bacterial, 71 archaeal, and 7204 viral genomes and annotated over 4,817,256 non-redundant protein-coding genes. We revealed patterns of feeding-driven microbial community dynamics along the goat GIT sites which were likely associated with gastrointestinal food digestion and absorption capabilities and disease risks, and identified an abundance of large intestine-enriched genera involved in plant fiber digestion. We quantified the effects of various factors affecting the distribution and abundance of methane-producing microbes including the GIT site, age, feeding style, and geography, and identified 68 virulent viruses targeting the methane producers via a comprehensive virus-bacterium/archaea interaction network. Together, our GMMC catalog provides functional insights of the goat GIT microbiota through microbiome-host interactions and paves the way to microbial interventions for better goat and eco-environmental qualities. Video Abstract.