Title |
A second generation framework for the analysis of microsatellites in expressed sequence tags and the development of EST-SSR markers for a conifer, Cryptomeria japonica
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Published in |
BMC Genomics, January 2012
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DOI | 10.1186/1471-2164-13-136 |
Pubmed ID | |
Authors |
Saneyoshi Ueno, Yoshinari Moriguchi, Kentaro Uchiyama, Tokuko Ujino-Ihara, Norihiro Futamura, Tetsuya Sakurai, Kenji Shinohara, Yoshihiko Tsumura |
Abstract |
Microsatellites or simple sequence repeats (SSRs) in expressed sequence tags (ESTs) are useful resources for genome analysis because of their abundance, functionality and polymorphism. The advent of commercial second generation sequencing machines has lead to new strategies for developing EST-SSR markers, necessitating the development of bioinformatic framework that can keep pace with the increasing quality and quantity of sequence data produced. We describe an open scheme for analyzing ESTs and developing EST-SSR markers from reads collected by Sanger sequencing and pyrosequencing of sugi (Cryptomeria japonica). |
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