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Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia

Overview of attention for article published in Irish Veterinary Journal, January 2017
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Title
Protein profiling of plasma proteins in dairy cows with subclinical hypocalcaemia
Published in
Irish Veterinary Journal, January 2017
DOI 10.1186/s13620-017-0082-0
Pubmed ID
Authors

Ziling Fan, Shi Shu, Chuchu Xu, Xinhuan Xiao, Gang Wang, Yunlong Bai, Cheng Xia, Ling Wu, Hongyou Zhang, Chuang Xu, Wei Yang

Abstract

Subclinical hypocalcaemia (SH) is an important metabolic disease in dairy cows that has a serious impact on production performance. The objective of this study was to investigate novel aspects of pathogenesis using proteomics technology to identify proteins that are differentially expressed in diseased and healthy animals. Dairy cows were divided into an SH group (T, n = 10) and a control group (C, n = 10) based on plasma calcium concentration. A total of 398 differentially expressed proteins were identified, of which 265 proteins were overlapped in the two parallel experiments. Of these, 24 differentially expressed proteins were statistically significant. Gene Ontology analysis yielded 74 annotations, including 7 cellular component, 55 biological process and 12 molecular function categories. Bioinformatics analysis indicated that calcium regulation, immune and inflammatory response, blood coagulation and complement pathway were all related to SH. Our iTRAQ/LC-MS/MS (isobaric tags for relative and absolute quantification/liquid chromatography-mass spectrometry/mass spectrometry) approach proved highly effective for plasma protein profiling of dairy cows with SH, and the results pave the way for further studies in this area.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 2 15%
Student > Master 2 15%
Student > Bachelor 2 15%
Librarian 1 8%
Other 1 8%
Other 2 15%
Unknown 3 23%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 38%
Veterinary Science and Veterinary Medicine 4 31%
Business, Management and Accounting 1 8%
Unknown 3 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2017.
All research outputs
#17,285,668
of 25,373,627 outputs
Outputs from Irish Veterinary Journal
#144
of 257 outputs
Outputs of similar age
#267,601
of 421,358 outputs
Outputs of similar age from Irish Veterinary Journal
#3
of 3 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 257 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,358 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.