Title |
Automated assay for screening the enzymatic release of reducing sugars from micronized biomass
|
---|---|
Published in |
Microbial Cell Factories, July 2010
|
DOI | 10.1186/1475-2859-9-58 |
Pubmed ID | |
Authors |
David Navarro, Marie Couturier, Gabriela Ghizzi Damasceno da Silva, Jean-Guy Berrin, Xavier Rouau, Marcel Asther, Christophe Bignon |
Abstract |
To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-throughput screening methods. Several methods have been devised all using microplates in the industrial SBS format. Although this size reduction and standardization has greatly improved the screening process, the published methods comprise one or more manual steps that seriously decrease throughput. Therefore, we worked to devise a screening method devoid of any manual steps. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | <1% |
Germany | 1 | <1% |
Italy | 1 | <1% |
Brazil | 1 | <1% |
Finland | 1 | <1% |
United Kingdom | 1 | <1% |
Japan | 1 | <1% |
Unknown | 130 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 36 | 26% |
Student > Ph. D. Student | 29 | 21% |
Student > Master | 17 | 12% |
Student > Bachelor | 10 | 7% |
Student > Doctoral Student | 8 | 6% |
Other | 24 | 18% |
Unknown | 13 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 77 | 56% |
Biochemistry, Genetics and Molecular Biology | 17 | 12% |
Chemistry | 7 | 5% |
Engineering | 6 | 4% |
Chemical Engineering | 3 | 2% |
Other | 11 | 8% |
Unknown | 16 | 12% |