Title |
Multiplex PCR followed by restriction length polymorphism analysis for the subtyping of bovine herpesvirus 5 isolates
|
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Published in |
BMC Veterinary Research, June 2013
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DOI | 10.1186/1746-6148-9-111 |
Pubmed ID | |
Authors |
Silvina Soledad Maidana, Cintia Débora Morano, Daniela Cianfrini, Fabrício Souza Campos, Paulo Michel Roehe, Bianca Siedler, Gabriel De Stefano, Axel Mauroy, Etienne Thiry, Sonia Alejandra Romera |
Abstract |
Several types and subtypes of bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) have been associated to different clinical conditions of cattle, making type/subtype differentiation essential to understand the pathogenesis and epidemiology of BoHV infections. BoHV-5 subtyping is currently carried out by BstEII restriction enzyme analysis (REA) of the complete virus genome. This method allowed the description of three subtypes, one of which is the most widespread while the remaining two have so far only been found in South America. The present work describes a multiplex PCR followed by REA for BoHV-5 subtyping. |
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United Kingdom | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Doctoral Student | 6 | 22% |
Student > Ph. D. Student | 5 | 19% |
Other | 3 | 11% |
Researcher | 3 | 11% |
Student > Bachelor | 1 | 4% |
Other | 3 | 11% |
Unknown | 6 | 22% |
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Veterinary Science and Veterinary Medicine | 4 | 15% |
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Medicine and Dentistry | 2 | 7% |
Other | 2 | 7% |
Unknown | 5 | 19% |