Title |
User guide for mapping-by-sequencing in Arabidopsis
|
---|---|
Published in |
Genome Biology, June 2013
|
DOI | 10.1186/gb-2013-14-6-r61 |
Pubmed ID | |
Authors |
Geo Velikkakam James, Vipul Patel, Karl JV Nordström, Jonas R Klasen, Patrice A Salomé, Detlef Weigel, Korbinian Schneeberger |
Abstract |
Mapping-by-sequencing combines genetic mapping with whole-genome sequencing in order to accelerate mutant identification. However, application of mapping-by-sequencing requires decisions on various practical settings on the experimental design that are not intuitively answered. Following an experimentally determined recombination landscape of Arabidopsis and next generation sequencing-specific biases, we simulated more than 400,000 mapping-by-sequencing experiments. This allowed us to evaluate a broad range of different types of experiments and to develop general rules for mapping-by-sequencing in Arabidopsis. Most importantly, this informs about the properties of different crossing scenarios, the number of recombinants and sequencing depth needed for successful mapping experiments. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 5 | 25% |
Germany | 4 | 20% |
United States | 3 | 15% |
Spain | 1 | 5% |
India | 1 | 5% |
Mexico | 1 | 5% |
Unknown | 5 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 11 | 55% |
Scientists | 7 | 35% |
Science communicators (journalists, bloggers, editors) | 2 | 10% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 4 | <1% |
Germany | 3 | <1% |
France | 3 | <1% |
United States | 3 | <1% |
China | 3 | <1% |
Sweden | 2 | <1% |
Netherlands | 2 | <1% |
India | 2 | <1% |
Belgium | 2 | <1% |
Other | 6 | 1% |
Unknown | 439 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 122 | 26% |
Student > Ph. D. Student | 111 | 24% |
Student > Master | 56 | 12% |
Student > Bachelor | 35 | 7% |
Professor | 24 | 5% |
Other | 60 | 13% |
Unknown | 61 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 296 | 63% |
Biochemistry, Genetics and Molecular Biology | 93 | 20% |
Computer Science | 5 | 1% |
Environmental Science | 2 | <1% |
Medicine and Dentistry | 2 | <1% |
Other | 8 | 2% |
Unknown | 63 | 13% |