Title |
Protein-restricted diet during pregnancy after insemination alters behavioral phenotypes of the progeny
|
---|---|
Published in |
Genes & Nutrition, January 2017
|
DOI | 10.1186/s12263-016-0550-2 |
Pubmed ID | |
Authors |
Tamio Furuse, Kunio Miyake, Takashi Kohda, Hideki Kaneda, Takae Hirasawa, Ikuko Yamada, Tomoko Kushida, Misho Kashimura, Kimio Kobayashi, Fumitoshi Ishino, Takeo Kubota, Shigeharu Wakana |
Abstract |
Epidemiological studies suggest that hyponutrition during the fetal period increases the risk of mental disorders such as attention deficit hyperactivity disorder and autism-spectrum disorder, which has been experimentally supported using animal models. However, previous experimental hyponutrition or protein-restricted (PR) diets affected stages other than the fetal stage, such as formation of the egg before insemination, milk composition during lactation, and maternal nursing behavior. We conducted in vitro fertilization and embryo transfer in mice and allowed PR diet and folic acid-supplemented PR diet to affect only fetal environments. Comprehensive phenotyping of PR and control-diet progenies showed moderate differences in fear/anxiety-like, novelty-seeking, and prosocial behaviors, irrespective of folic-acid supplementation. Changes were also detected in gene expression and genomic methylation in the brain. These results suggest that epigenetic factors in the embryo/fetus influence behavioral and epigenetic phenotypes of progenies. Significant epigenetic alterations in the brains of the progenies induced by the maternal-protein restriction were observed in the present study. To our knowledge, this is first study to evaluate the effect of maternal hyponutrition on behavioral phenotypes using reproductive technology. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 1 | 1% |
Unknown | 66 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 12 | 18% |
Student > Master | 9 | 13% |
Student > Bachelor | 9 | 13% |
Researcher | 7 | 10% |
Student > Doctoral Student | 4 | 6% |
Other | 9 | 13% |
Unknown | 17 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Psychology | 12 | 18% |
Biochemistry, Genetics and Molecular Biology | 9 | 13% |
Medicine and Dentistry | 7 | 10% |
Nursing and Health Professions | 4 | 6% |
Agricultural and Biological Sciences | 4 | 6% |
Other | 13 | 19% |
Unknown | 18 | 27% |