Title |
Changes in the respiratory microbiome during acute exacerbations of idiopathic pulmonary fibrosis
|
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Published in |
Respiratory Research, February 2017
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DOI | 10.1186/s12931-017-0511-3 |
Pubmed ID | |
Authors |
Philip L. Molyneaux, Michael J Cox, Athol U. Wells, Ho Cheol Kim, Wonjun Ji, William O. C. Cookson, Miriam F. Moffatt, Dong Soon Kim, Toby M. Maher |
Abstract |
Acute exacerbations of idiopathic pulmonary fibrosis (AE-IPF) have been defined as events of clinically significant respiratory deterioration with an unidentifiable cause. They carry a significant mortality and morbidity and while their exact pathogenesis remains unclear, the possibility remains that hidden infection may play a role. The aim of this pilot study was to determine whether changes in the respiratory microbiota occur during an AE-IPF. Bacterial DNA was extracted from bronchoalveolar lavage from patients with stable IPF and those experiencing an AE-IPF. A hyper-variable region of the 16S ribosomal RNA gene (16S rRNA) was amplified, quantified and pyrosequenced. Culture independent techniques demonstrate AE-IPF is associated with an increased BAL bacterial burden compared to stable disease and highlight shifts in the composition of the respiratory microbiota during an AE-IPF. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 4 | 22% |
Sweden | 2 | 11% |
United States | 2 | 11% |
Australia | 1 | 6% |
Spain | 1 | 6% |
Canada | 1 | 6% |
Venezuela, Bolivarian Republic of | 1 | 6% |
Unknown | 6 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 14 | 78% |
Scientists | 3 | 17% |
Practitioners (doctors, other healthcare professionals) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | <1% |
Unknown | 134 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 21 | 16% |
Other | 14 | 10% |
Student > Ph. D. Student | 14 | 10% |
Student > Master | 13 | 10% |
Student > Postgraduate | 12 | 9% |
Other | 31 | 23% |
Unknown | 30 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 50 | 37% |
Agricultural and Biological Sciences | 13 | 10% |
Immunology and Microbiology | 11 | 8% |
Biochemistry, Genetics and Molecular Biology | 9 | 7% |
Social Sciences | 2 | 1% |
Other | 12 | 9% |
Unknown | 38 | 28% |