Title |
Analysis of hairpin RNA transgene-induced gene silencing in Fusarium oxysporum
|
---|---|
Published in |
Silence, July 2013
|
DOI | 10.1186/1758-907x-4-3 |
Pubmed ID | |
Authors |
Ulrike Schumann, Neil A Smith, Kemal Kazan, Michael Ayliffe, Ming-Bo Wang |
Abstract |
Hairpin RNA (hpRNA) transgenes can be effective at inducing RNA silencing and have been exploited as a powerful tool for gene function analysis in many organisms. However, in fungi, expression of hairpin RNA transcripts can induce post-transcriptional gene silencing, but in some species can also lead to transcriptional gene silencing, suggesting a more complex interplay of the two pathways at least in some fungi. Because many fungal species are important pathogens, RNA silencing is a powerful technique to understand gene function, particularly when gene knockouts are difficult to obtain. We investigated whether the plant pathogenic fungus Fusarium oxysporum possesses a functional gene silencing machinery and whether hairpin RNA transcripts can be employed to effectively induce gene silencing. |
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