Title |
H1N1 influenza vaccination in HIV-infected women on effective antiretroviral treatment did not induce measurable antigen-driven proliferation of the HIV-1 proviral reservoir
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Published in |
AIDS Research and Therapy, February 2017
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DOI | 10.1186/s12981-017-0135-1 |
Pubmed ID | |
Authors |
Thor A. Wagner, Hannah C. Huang, Christen E. Salyer, Kelly M. Richardson, Adriana Weinberg, Sharon Nachman, Lisa M. Frenkel |
Abstract |
Antigen-induced activation and proliferation of HIV-1-infected cells is hypothesized to be a mechanism of HIV persistence during antiretroviral therapy. The objective of this study was to determine if proliferation of H1N1-specific HIV-infected cells could be detected following H1N1 vaccination. This study utilized cryopreserved PBMC from a previously conducted trial of H1N1 vaccination in HIV-infected pregnant women. HIV-1 DNA concentrations and 437 HIV-1 C2V5 env DNA sequences were analyzed from ten pregnant women on effective antiretroviral therapy, before and 21 days after H1N1 influenza vaccination. HIV-1 DNA concentration did not change after vaccination (median pre- vs. post-vaccination: 95.77 vs. 41.28 copies/million PBMC, p = .37). Analyses of sequences did not detect evidence of HIV replication or proliferation of infected cells. Antigenic stimulation during effective ART did not have a detectable effect on the genetic makeup of the HIV-1 DNA reservoir. Longitudinal comparison of the amount and integration sites of HIV-1 in antigen-specific cells to chronic infections (such as herpesviruses) may be needed to definitively evaluate whether antigenic stimulation induces proliferation of HIV-1 infected cells. |
X Demographics
Geographical breakdown
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 21% |
Researcher | 6 | 18% |
Student > Master | 4 | 12% |
Student > Bachelor | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Other | 3 | 9% |
Unknown | 8 | 24% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 9 | 27% |
Biochemistry, Genetics and Molecular Biology | 3 | 9% |
Agricultural and Biological Sciences | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Social Sciences | 2 | 6% |
Other | 1 | 3% |
Unknown | 13 | 39% |