Title |
Virus replicon particle based Chikungunya virus neutralization assay using Gaussia luciferase as readout
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Published in |
Virology Journal, July 2013
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DOI | 10.1186/1743-422x-10-235 |
Pubmed ID | |
Authors |
Sabine Gläsker, Aleksei Lulla, Valeria Lulla, Therese Couderc, Jan Felix Drexler, Peter Liljeström, Marc Lecuit, Christian Drosten, Andres Merits, Beate Mareike Kümmerer |
Abstract |
Chikungunya virus (CHIKV) has been responsible for large epidemic outbreaks causing fever, headache, rash and severe arthralgia. So far, no specific treatment or vaccine is available. As nucleic acid amplification can only be used during the viremic phase of the disease, serological tests like neutralization assays are necessary for CHIKV diagnosis and for determination of the immune status of a patient. Furthermore, neutralization assays represent a useful tool to validate the efficacy of potential vaccines. As CHIKV is a BSL3 agent, neutralization assays with infectious virus need to be performed under BSL3 conditions. Our aim was to develop a neutralization assay based on non-infectious virus replicon particles (VRPs). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 2% |
Turkey | 1 | 1% |
Unknown | 86 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 25% |
Student > Ph. D. Student | 13 | 15% |
Student > Bachelor | 7 | 8% |
Other | 7 | 8% |
Student > Master | 7 | 8% |
Other | 13 | 15% |
Unknown | 20 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 21 | 24% |
Biochemistry, Genetics and Molecular Biology | 15 | 17% |
Immunology and Microbiology | 13 | 15% |
Medicine and Dentistry | 3 | 3% |
Engineering | 3 | 3% |
Other | 13 | 15% |
Unknown | 21 | 24% |