Title |
GLiMMPS: robust statistical model for regulatory variation of alternative splicing using RNA-seq data
|
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Published in |
Genome Biology, July 2013
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DOI | 10.1186/gb-2013-14-7-r74 |
Pubmed ID | |
Authors |
Keyan Zhao, Zhi-xiang Lu, Juw Won Park, Qing Zhou, Yi Xing |
Abstract |
To characterize the genetic variation of alternative splicing, we develop GLiMMPS, a robust statistical method for detecting splicing quantitative trait loci (sQTLs) from RNA-seq data. GLiMMPS takes into account the individual variation in sequencing coverage and the noise prevalent in RNA-seq data. Analyses of simulated and real RNA-seq datasets demonstrate that GLiMMPS outperforms competing statistical models. Quantitative RT-PCR tests of 26 randomly selected GLiMMPS sQTLs yielded a validation rate of 100%. As population-scale RNA-seq studies become increasingly affordable and popular, GLiMMPS provides a useful tool for elucidating the genetic variation of alternative splicing in humans and model organisms. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 3 | 27% |
Germany | 1 | 9% |
Australia | 1 | 9% |
United Kingdom | 1 | 9% |
France | 1 | 9% |
Montenegro | 1 | 9% |
Unknown | 3 | 27% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 6 | 55% |
Members of the public | 4 | 36% |
Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 3% |
Korea, Republic of | 1 | <1% |
Germany | 1 | <1% |
Belgium | 1 | <1% |
Sweden | 1 | <1% |
Unknown | 137 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 54 | 37% |
Researcher | 40 | 27% |
Student > Master | 9 | 6% |
Professor > Associate Professor | 7 | 5% |
Student > Doctoral Student | 6 | 4% |
Other | 18 | 12% |
Unknown | 12 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 77 | 53% |
Biochemistry, Genetics and Molecular Biology | 28 | 19% |
Computer Science | 12 | 8% |
Mathematics | 3 | 2% |
Medicine and Dentistry | 3 | 2% |
Other | 6 | 4% |
Unknown | 17 | 12% |