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Methods for microbial DNA extraction from soil for PCR amplification

Overview of attention for article published in Biological Procedures Online, May 1998
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#31 of 192)
  • High Attention Score compared to outputs of the same age (85th percentile)

Mentioned by

patent
12 patents
video
1 YouTube creator

Citations

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223 Dimensions

Readers on

mendeley
804 Mendeley
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Title
Methods for microbial DNA extraction from soil for PCR amplification
Published in
Biological Procedures Online, May 1998
DOI 10.1251/bpo6
Pubmed ID
Authors

C. Yeates, M. R. Gillings, A. D. Davison, N. Altavilla, D. A. Veal

Abstract

Amplification of DNA from soil is often inhibited by co-purified contaminants. A rapid, inexpensive, large-scale DNA extraction method involving minimal purification has been developed that is applicable to various soil types (1). DNA is also suitable for PCR amplification using various DNA targets. DNA was extracted from 100g of soil using direct lysis with glass beads and SDS followed by potassium acetate precipitation, polyethylene glycol precipitation, phenol extraction and isopropanol precipitation. This method was compared to other DNA extraction methods with regard to DNA purity and size.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 804 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 8 <1%
United States 6 <1%
Brazil 5 <1%
Spain 4 <1%
Philippines 3 <1%
Indonesia 2 <1%
South Africa 2 <1%
Malaysia 2 <1%
Canada 2 <1%
Other 18 2%
Unknown 752 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 163 20%
Student > Bachelor 136 17%
Researcher 131 16%
Student > Master 127 16%
Student > Doctoral Student 43 5%
Other 107 13%
Unknown 97 12%
Readers by discipline Count As %
Agricultural and Biological Sciences 383 48%
Biochemistry, Genetics and Molecular Biology 117 15%
Environmental Science 63 8%
Immunology and Microbiology 25 3%
Engineering 25 3%
Other 78 10%
Unknown 113 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2023.
All research outputs
#5,338,984
of 25,373,627 outputs
Outputs from Biological Procedures Online
#31
of 192 outputs
Outputs of similar age
#4,821
of 33,392 outputs
Outputs of similar age from Biological Procedures Online
#1
of 2 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 78th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 192 research outputs from this source. They receive a mean Attention Score of 4.6. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 33,392 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them